Primary funding for this project comes from the National Institute of General Medical Sciences(NIGMS) , a division of the National Institutes of Health (NIH). Additional financial support for NE-CAT comes from the member institutions.
National Institutes of Health
The Northeastern Collaborative Access Team (NE-CAT) is funded by its member institutions and the National Institute of General Medical Sciences (NIGMS) to operate synchrotron X-ray beamlines to address technically challenging problems in structural biology. These beamlines are an important resource for NE-CAT's institutional members as well as the national research community. NE-CAT provides 50% of the available beam time to the national user community. These beamlines have been developed using the extremely high-brilliance X-ray sources provided by the novel dual-canted undulators of the Advanced Photon Source (APS) at the Argonne National Laboratory. The optical trains for NE-CAT’s two canted-undulator beamlines are schematically shown below.
Both undulator beamlines, 24-ID-C and 24-ID-E, are in full operation providing exceptionally stable well collimated X-ray beams and are optimized for microdiffraction crystallographic research. The 24-ID-C beamline is a variable-energy beamline providing X-rays from 6.5 to 20 keV. The 24-ID-E beamline is a fixed–energy microdiffraction beamline providing X-rays at 12.66 keV. Detailed descriptions of the beamlines can be found under “Facilities”.
The user end stations for all the beamlines are fully equipped with state-of-the-art instrumentation for its users. Both undulator beamlines are equipped with MD2 microdiffractometers. The MD2 provides exceptionally well collimated beams from 5 microns to 100 microns in diameter and has sample visualization systems capable of visualizing micron-sized crystals. For data collection, both beamlines are equipped with very large-area CCD-based ADSC Quantum 315 detectors connected to a data storage system currently with a capacity of 39TB. The data acquisition systems are designed for very fast data acquisition, capable of taking exposures as short as 250 msec, with a dead time of 2 sec for un-binned images and 1 sec for binned images. Advanced Light Source (ALS)-type robotic sample auto-mount systems are available on both beamlines to automatically mount and dismount crystals. This robotic system makes screening large numbers of crystals much faster and easier. Users of the beamlines are supported by experienced crystallographers and have access to a full suite of on-line and off-line data processing software to analyze their data and solve macromolecular structures. A fully equipped chemistry laboratory is also available for users. Shown below are photographs of the two undulator-based user endstations.
February 21, 2014
Cheng, W., and Li, W. (2014) Structural insights into ubiquinone biosynthesis in membranes, Science 343, 878-881.
January 23, 2014
Zhou, X., Levin, E. J., Pan, Y., McCoy, J. G., Sharma, R., Kloss, B., Bruni, R., Quick, M., and Zhou, M. (2014) Structural basis of the alternating-access mechanism in a bile acid transporter, Nature 505, 569-573.
December 12, 2013
Geng, Y., Bush, M., Mosyak, L., Wang, F., and Fan, Q. R. (2013) Structural mechanism of ligand activation in human GABAB receptor, Nature 504, 254-259.
November 7, 2013
Penmatsa, A., Wang, K. H., and Gouaux, E. (2013) X-ray structure of dopamine transporter elucidates antidepressant mechanism, Nature 503, 85-90.
Wang, H., Goehring, A., Wang, K. H., Penmatsa, A., Ressler, R., and Gouaux, E. (2013) Structural basis for action by diverse antidepressants on biogenic amine transporters, Nature 503, 141-145. Also highlighted in Chemical and Engineering News
August 30, 2013
Chung, B. C., Zhao, J., Gillespie, R. A., Kwon, D. Y., Guan, Z., Hong, J., Zhou, P., and Lee, S. Y. (2013) Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis, Science 341, 1012-1016.
August 22, 2013
Simanshu, D. K., Kamlekar, R. K., Wijesinghe, D. S., Zou, X., Zhai, X., Mishra, S. K., Molotkovsky, J. G., Malinina, L., Hinchcliffe, E. H., Chalfant, C. E., Brown, R. E., and Patel, D. J. (2013) Non-vesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids, Nature 500, 463-467.
August 15, 2013
Zhang, J., and Ferre-D'Amare, A. R. (2013) Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA, Nature 500, 363-366.
Also, highlighted in News and Views
August 15, 2013
Lomakin, I. B., and Steitz, T. A. (2013) The initiation of mammalian protein synthesis and mRNA scanning mechanism, Nature 500, 307-311.
June 20, 2013
Rivkin, E., Almeida, S. M., Ceccarelli, D. F., Juang, Y. C., Maclean, T. A., Srikumar, T., Huang, H., Dunham, W. H., Fukumura, R., Xie, G., Gondo, Y., Raught, B., Gingras, A. C., Sicheri, F., and Cordes, S. P. (2013) The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis, Nature 498, 318-324.
June 20, 2013
Law, J. A., Du, J., Hale, C. J., Feng, S., Krajewski, K., Palanca, A. M. S., Strahl, B. D., Patel, D. J., and Jacobsen, S. E. (2013) Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1, Nature 498, 385-389.
May 9, 2013
Yang, H., Rudge, D. G., Koos, J. D., Vaidialingam, B., Yang, H. J., and Pavletich, N. P. (2013) mTOR kinase structure, mechanism and regulation, Nature 497, 217-223.
April 25, 2013
Stinson, Benjamin M., Nager, Andrew R., Glynn, Steven E., Schmitz, Karl R., Baker, Tania A., and Sauer, Robert T. (2013) Nucleotide Binding and Conformational Switching in the Hexameric Ring of a AAA+ Machine, Cell 153, 628-639.
February 14, 2013
Oh, Y. S., Gao, P., Lee, K. W., Ceglia, I., Seo, J. S., Zhang, X., Ahn, J. H., Chait, B. T., Patel, D. J., Kim, Y., and Greengard, P. (2013) SMARCA3, a Chromatin-Remodeling Factor, Is Required for p11-Dependent Antidepressant Action, Cell 152, 831-843.
January 31, 2013
Weixlbaumer, A., Leon, K., Landick, R., and Darst, S. A. (2013) Structural Basis of Transcriptional Pausing in Bacteria, Cell 152, 431-441.
January 10, 2013
Strugatsky, D., McNulty, R., Munson, K., Chen, C. K., Soltis, S. M., Sachs, G., and Luecke, H. (2013) Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori, Nature 493, 255–258.
© 2005 Northeastern Collaborative Access Team
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